Perturbation-Aware Diffusion-Guided Hybrid Segmentation for Robust and Annotation-Efficient Plant Stress Phenotyping

Semantic segmentation in agricultural imagery is often evaluated under in-domain protocols, yet practical deployment requires robustness to appearance perturbations, limited annotations, and cross domain shift. This paper presents a diffusion-guided hybrid segmentation framework in which U-Net, DeepLabV3+, and SegFormer backbones generate coarse masks that are refined by Denoising Diffusion Probabilistic Models (DDPM), latent diffusion, or semantic-guided diffusion. The framework is evaluated through a 3x3 architectural screening study on PlantSegV3, followed by boundary-constrained optimization, perturbation-guided retraining, low-data evaluation, constrained hyperparameter screening, and controlled cross-domain adaptation. On PlantSegV3, the best selected hybrid model achieves 71.83% refined mean Intersection-over-Union (mIoU) and 26.10% refined Boundary-F1, and the selected models remain stable under substantially reduced supervision, demonstrating strong annotation efficiency. Perturbation analysis identifies grayscale conversion, fog, coarse dropout, and shadow as the most disruptive appearance shifts, and the resulting augmentation policy substantially improves robustness during retraining. The adapted models further show effective transfer to external agricultural datasets under limited target supervision, indicating that diffusion refinement and boundary-aware optimization provide transferable structural priors. Overall, the results show that carefully matched backbone-refiner pairings, combined with perturbation-aware retraining, can improve structural delineation and robustness under realistic resource and distribution constraints.