Frequency-Aware Dual-Stream Learning for Balanced Realism and Fidelity in Electron Microscopy Imaging

Electron microscopy enables nanoscale cellular visualization but faces a trade-off between imaging resolution and acquisition speed. Existing learning-based methods rely on single-stream architectures that struggle to balance perceptual realism and quantitative fidelity, either over-smoothing details or generating unrealistic hallucinations. This work introduces a frequency-adaptive dual-stream architecture to resolve this conflict. Using discrete wavelet transform, we decompose images into low-frequency structures and high-frequency details, then employ a conditional diffusion model for realistic global synthesis and a transformer network for precise detail recovery. Experiments on the EMDiffuse dataset show the method achieves superior LPIPS and resolution ratio, substantially outperforming existing approaches. The method also shows strong generalization across diverse biological samples, supporting fast and reliable electron microscopy imaging for structural biology and nanotechnology applications. The source code and associated dataset are publicly available to facilitate further research.