mlsanityData to 14 Oct 2026

EAP4EMSIG -- Enhancing Event-Driven Microscopy for Microfluidic Single-Cell Analysis

arXiv 2504.00047 ↗First posted 30 Mar 2025q-bio.QM cs.AI cs.CVcited by 0

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Abstract (arXiv)

Microfluidic Live-Cell Imaging (MLCI) yields data on microbial cell factories. However, continuous acquisition is challenging as high-throughput experiments often lack real-time insights, delaying responses to stochastic events. We introduce three components in the Experiment Automation Pipeline for Event-Driven Microscopy to Smart Microfluidic Single-Cell Analysis (EAP4EMSIG): a fast, accurate Multi-Layer Perceptron (MLP)-based autofocusing method predicting the focus offset, an evaluation of real-time segmentation methods and a real-time data analysis dashboard. Our MLP-based autofocusing achieves a Mean Absolute Error (MAE) of 0.105 μm with inference times from 87 ms. Among eleven evaluated Deep Learning (DL) segmentation methods, Cellpose reached a Panoptic Quality (PQ) of 93.36 %, while a distance-based method was fastest (121 ms, Panoptic Quality 93.02 %).

Key facts. No citing papers in the map yet (data through 14 Oct 2026). Builds on 21 of its 74 references that are in the map. First posted on arXiv 30 Mar 2025.

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21 of 74 references.

References · 21

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9 more
  1. 2021CFPNet-M: A Light-Weight Encoder-Decoder Based Network for Multimodal Biomedical Image Real-Time Segmentationcited by 11
  2. 2021Task Fingerprinting for Meta Learning in Biomedical Image Analysiscited by 6
  3. 2024Tracking one-in-a-million: Large-scale benchmark for microbial single-cell tracking with experiment-aware robustness metricscited by 6
  4. 2020Autofocusing technologies for whole slide imaging and automated microscopycited by 5
  5. 2023MLOps for Scarce Image Data: A Use Case in Microscopic Image Analysiscited by 4
  6. 2024Beyond Knowledge Silos: Task Fingerprinting for Democratization of Medical Imaging AIcited by 2
  7. 2023AI-based automated active learning for discovery of hidden dynamic processes: A use case in light microscopycited by 1
  8. 2023Security Fence Inspection at Airports Using Object Detectioncited by 1
  9. 2024EAP4EMSIG -- Experiment Automation Pipeline for Event-Driven Microscopy to Smart Microfluidic Single-Cells Analysiscited by 1

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