Laryngeal Structure Segmentation in High-Speed Videoendoscopy Using Deep Learning

Laryngeal high-speed videoendoscopy (HSV) offers an effective means of observing the motion of different laryngeal structures along with vibratory behaviors of the vocal folds under various voicing conditions. Segmentation of laryngeal tissues enables analysis of different tissue structures and their dynamics, helping characterize the involvement of laryngeal muscles in voice production. Given the large number of HSV frames, automating this task is imperative. While deep learning-based methods have been implemented in previous studies to segment laryngeal structures, they have not been applied to HSV data during connected speech, which poses significant challenges due to excessive tissue movements and image quality limitations associated with fiberoptic image acquisition. The application of deep learning to connected speech data is critical for capturing nonstationary laryngeal behaviors and identifying anomalous patterns associated with voice disorders. The present study aims to address these gaps by training U-Net models to detect the aryepiglottic folds and arytenoid cartilages, vocal folds, epiglottis, and glottal area, using HSV data from both sustained vowel phonation and connected speech obtained from normophonic and disordered voices. Image pre-processing techniques, including noise removal and histogram equalization, were applied to improve the quality of the training HSV images and enhance network performance. Finally, to evaluate the accuracy and reliability of the networks, quantitative performance metrics were used alongside qualitative visual inspection of the test images. The high performance of the developed networks, with overall accuracies exceeding 95%, establishes their potential as reliable tools for automated laryngeal image analysis, quantitative characterization of laryngeal dynamics, and future detection of anomalous laryngeal behaviors in clinical settings.