From Fibers to Cells: Fourier-Based Registration Enables Virtual Cresyl Violet Staining From 3D Polarized Light Imaging

Comprehensive assessment of the various aspects of the brain's microstructure requires the use of complementary imaging techniques. This includes measuring the spatial distribution of cell bodies (cytoarchitecture) and nerve fibers (myeloarchitecture). The gold standard for cytoarchitectonic analysis is light microscopic imaging of cell-body stained tissue sections. To reveal the 3D orientations of nerve fibers, 3D Polarized Light Imaging (3D-PLI) has been introduced, a method that is label-free and allows subsequent staining of sections after 3D-PLI measurement. By post-staining for cell bodies, a direct link between fiber- and cytoarchitecture can potentially be established in the same section. However, inevitable distortions introduced during the staining process make a costly nonlinear and cross-modal registration necessary in order to study the detailed relationships between cells and fibers in the images. In addition, the complexity of processing histological sections for post-staining only allows for a limited number of such samples. In this work, we take advantage of deep learning methods for image-to-image translation to generate a virtual staining of 3D-PLI that is spatially aligned at the cellular level. We use a supervised setting, building on a unique dataset of brain sections, to which Cresyl violet staining has been applied after 3D-PLI measurement. To ensure high correspondence between both modalities, we address the misalignment of training data using Fourier-based registration. In this way, registration can be efficiently calculated during training for local image patches of target and predicted staining. We demonstrate that the proposed method can predict a Cresyl violet staining from 3D-PLI, resulting in a virtual staining that exhibits plausible patterns of cell organization in gray matter, with larger cell bodies being localized at their expected positions.