APSeg: Auto-Prompt Model with Acquired and Injected Knowledge for Nuclear Instance Segmentation and Classification

Nuclear instance segmentation and classification provide critical quantitative foundations for digital pathology diagnosis. With the advent of the foundational Segment Anything Model (SAM), the accuracy and efficiency of nuclear segmentation have improved significantly. However, SAM imposes a strong reliance on precise prompts, and its class-agnostic design renders its classification results entirely dependent on the provided prompts. Therefore, we focus on generating prompts with more accurate localization and classification and propose APSeg, Auto-Prompt model with acquired and injected knowledge for nuclear instance Segmentation and classification. APSeg incorporates two knowledge-aware modules: (1) Distribution-Guided Proposal Offset Module (DG-POM), which learns distribution knowledge through density map guided, and (2) Category Knowledge Semantic Injection Module (CK-SIM), which injects morphological knowledge derived from category descriptions. We conducted extensive experiments on the PanNuke and CoNSeP datasets, demonstrating the effectiveness of our approach. The code will be released upon acceptance.