# DART: A design-aware microfluidic chip paradigm for real-time live-cell image analysis

arXiv: https://arxiv.org/abs/2606.18523 · HTML: https://mlsanity.com/paper/2606.18523/

## Key facts

- No citing papers in the map yet (data through 14 Oct 2026).
- Builds on 6 of its 41 references that are in the map.
- First posted on arXiv 16 Jun 2026.

## Builds on (most cited first, 6 of 6)

- [SAM 2: Segment Anything in Images and Videos](https://mlsanity.com/paper/2408.00714/) (2024)
- [YOLOv11: An Overview of the Key Architectural Enhancements](https://mlsanity.com/paper/2410.17725/) (2024)
- [Kornia: an Open Source Differentiable Computer Vision Library for PyTorch](https://mlsanity.com/paper/1910.02190/) (2019)
- [YOLO26: Key Architectural Enhancements and Performance Benchmarking for Real-Time Object Detection](https://mlsanity.com/paper/2509.25164/) (2025)
- [What is YOLOv8: An In-Depth Exploration of the Internal Features of the Next-Generation Object Detector](https://mlsanity.com/paper/2408.15857/) (2024)
- [What is YOLOv5: A deep look into the internal features of the popular object detector](https://mlsanity.com/paper/2407.20892/) (2024)

## Abstract (arXiv)

High-throughput microfluidic live-cell imaging generates rich single-cell data. Yet semi-automated procedures for locating regions of interest (RoIs), each containing one cell population, and removing surrounding microfluidic structures from recorded images, scale with the number of RoIs. This prevents real-time image analysis and delays time-to-insight by hours to days. We introduce the Design-Aware and Real-Time capable (DART) paradigm for microfluidic cultivation chips, which aligns the CAD blueprint with the physical chip and thereby enables throughput-independent localization of all RoIs and fully automated image processing across diverse RoI geometries and chip layouts. DART establishes this alignment through embedded fiducial markers and deep-learning-based marker detection. We validate DART using the Swiss Army Knife chip, which combines eight structurally distinct RoI designs across 1164 RoI locations. DART localizes all RoIs in five minutes, removes microfluidic structures from raw microscopy images in 40 ms, and performs fully automated image analysis, including cell segmentation, in under 1.1 s per image. Together, these capabilities establish DART as an end-to-end hardware-software paradigm with real-time-capable analysis that paves the way toward closed-loop and outcome-driven smart microscopy.