# PGVMS: A Prompt-Guided Unified Framework for Virtual Multiplex IHC Staining with Pathological Semantic Learning

arXiv: https://arxiv.org/abs/2602.23292 · HTML: https://mlsanity.com/paper/2602.23292/

## Key facts

- Cited by 1 papers in the map (data through 14 Oct 2026).
- Builds on 17 of its 31 references that are in the map.
- First posted on arXiv 26 Feb 2026.

## Builds on (most cited first, 17 of 17)

- [Learning Transferable Visual Models From Natural Language Supervision](https://mlsanity.com/paper/2103.00020/) (2021)
- [Representation Learning with Contrastive Predictive Coding](https://mlsanity.com/paper/1807.03748/) (2018)
- [Adding Conditional Control to Text-to-Image Diffusion Models](https://mlsanity.com/paper/2302.05543/) (2023)
- [Image-to-Image Translation with Conditional Adversarial Networks](https://mlsanity.com/paper/1611.07004/) (2016)
- [Contrastive Learning for Unpaired Image-to-Image Translation](https://mlsanity.com/paper/2007.15651/) (2020)
- [Denoising Diffusion Bridge Models](https://mlsanity.com/paper/2309.16948/) (2023)
- [Unpaired Image-to-Image Translation via Neural Schr"odinger Bridge](https://mlsanity.com/paper/2305.15086/) (2023)
- [Augmented CycleGAN: Learning Many-to-Many Mappings from Unpaired Data](https://mlsanity.com/paper/1802.10151/) (2018)
- [BCI: Breast Cancer Immunohistochemical Image Generation through Pyramid Pix2pix](https://mlsanity.com/paper/2204.11425/) (2022)
- [Adaptive Supervised PatchNCE Loss for Learning H&E-to-IHC Stain Translation with Inconsistent Groundtruth Image Pairs](https://mlsanity.com/paper/2303.06193/) (2023)
- [Pathological Semantics-Preserving Learning for H&E-to-IHC Virtual Staining](https://mlsanity.com/paper/2407.03655/) (2024)
- [Predicting Ki67, ER, PR, and HER2 Statuses from H&E-stained Breast Cancer Images](https://mlsanity.com/paper/2308.01982/) (2023)
- [The ACROBAT 2022 Challenge: Automatic Registration Of Breast Cancer Tissue](https://mlsanity.com/paper/2305.18033/) (2023)
- [VIMs: Virtual Immunohistochemistry Multiplex staining via Text-to-Stain Diffusion Trained on Uniplex Stains](https://mlsanity.com/paper/2407.19113/) (2024)
- [Unsupervised Image-to-Image Translation Using Domain-Specific Variational Information Bound](https://mlsanity.com/paper/1811.11979/) (2018)
- [Breast Cancer Immunohistochemical Image Generation: a Benchmark Dataset and Challenge Review](https://mlsanity.com/paper/2305.03546/) (2023)
- [Unpaired Multi-Domain Histopathology Virtual Staining using Dual Path Prompted Inversion](https://mlsanity.com/paper/2412.11106/) (2024)

## Built on by (most cited first, 1 of 1)

- [ContiStain: Cross-Domain Relation-Preserving Distillation for Continual Multi-Domain Virtual IHC Staining](https://mlsanity.com/paper/2607.03851/) (2026)

## Abstract (arXiv)

Immunohistochemical (IHC) staining enables precise molecular profiling of protein expression, with over 200 clinically available antibody-based tests in modern pathology. However, comprehensive IHC analysis is frequently limited by insufficient tissue quantities in small biopsies. Therefore, virtual multiplex staining emerges as an innovative solution to digitally transform H&E images into multiple IHC representations, yet current methods still face three critical challenges: (1) inadequate semantic guidance for multi-staining, (2) inconsistent distribution of immunochemistry staining, and (3) spatial misalignment across different stain modalities. To overcome these limitations, we present a prompt-guided framework for virtual multiplex IHC staining using only uniplex training data (PGVMS). Our framework introduces three key innovations corresponding to each challenge: First, an adaptive prompt guidance mechanism employing a pathological visual language model dynamically adjusts staining prompts to resolve semantic guidance limitations (Challenge 1). Second, our protein-aware learning strategy (PALS) maintains precise protein expression patterns by direct quantification and constraint of protein distributions (Challenge 2). Third, the prototype-consistent learning strategy (PCLS) establishes cross-image semantic interaction to correct spatial misalignments (Challenge 3).