# EMGauss: Continuous Slice-to-3D Reconstruction via Dynamic Gaussian Modeling in Volume Electron Microscopy

arXiv: https://arxiv.org/abs/2512.06684 · HTML: https://mlsanity.com/paper/2512.06684/

## Key facts

- No citing papers in the map yet (data through 14 Oct 2026).
- Builds on 13 of its 43 references that are in the map.
- First posted on arXiv 7 Dec 2025.

## Builds on (most cited first, 13 of 13)

- [PyTorch: An Imperative Style, High-Performance Deep Learning Library](https://mlsanity.com/paper/1912.01703/) (2019)
- [3D Gaussian Splatting for Real-Time Radiance Field Rendering](https://mlsanity.com/paper/2308.04079/) (2023)
- [Instant Neural Graphics Primitives with a Multiresolution Hash Encoding](https://mlsanity.com/paper/2201.05989/) (2022)
- [SAM 2: Segment Anything in Images and Videos](https://mlsanity.com/paper/2408.00714/) (2024)
- [4D Gaussian Splatting for Real-Time Dynamic Scene Rendering](https://mlsanity.com/paper/2310.08528/) (2023)
- [Deformable 3D Gaussians for High-Fidelity Monocular Dynamic Scene Reconstruction](https://mlsanity.com/paper/2309.13101/) (2023)
- [Noise2Void - Learning Denoising from Single Noisy Images](https://mlsanity.com/paper/1811.10980/) (2018)
- [GaussianImage: 1000 FPS Image Representation and Compression by 2D Gaussian Splatting](https://mlsanity.com/paper/2403.08551/) (2024)
- [GaussianSR: High Fidelity 2D Gaussian Splatting for Arbitrary-Scale Image Super-Resolution](https://mlsanity.com/paper/2407.18046/) (2024)
- [Large Images are Gaussians: High-Quality Large Image Representation with Levels of 2D Gaussian Splatting](https://mlsanity.com/paper/2502.09039/) (2025)
- [Deep Learning for Isotropic Super-Resolution from Non-Isotropic 3D Electron Microscopy](https://mlsanity.com/paper/1706.03142/) (2017)
- [DiffuseIR:Diffusion Models For Isotropic Reconstruction of 3D Microscopic Images](https://mlsanity.com/paper/2306.12109/) (2023)
- [Z-upscaling: Optical Flow Guided Frame Interpolation for Isotropic Reconstruction of 3D EM Volumes](https://mlsanity.com/paper/2410.07043/) (2024)

## Abstract (arXiv)

Volume electron microscopy (vEM) enables nanoscale 3D imaging of biological structures but remains constrained by acquisition trade-offs, leading to anisotropic volumes with limited axial resolution. Existing deep learning methods seek to restore isotropy by leveraging lateral priors, yet their assumptions break down for morphologically anisotropic structures. We present EMGauss, a general framework for 3D reconstruction from planar scanned 2D slices with applications in vEM, which circumvents the inherent limitations of isotropy-based approaches. Our key innovation is to reframe slice-to-3D reconstruction as a 3D dynamic scene rendering problem based on Gaussian splatting, where the progression of axial slices is modeled as the temporal evolution of 2D Gaussian point clouds. To enhance fidelity in data-sparse regimes, we incorporate a Teacher-Student bootstrapping mechanism that uses high-confidence predictions on unobserved slices as pseudo-supervisory signals. Compared with diffusion- and GAN-based reconstruction methods, EMGauss substantially improves interpolation quality, enables continuous slice synthesis, and eliminates the need for large-scale pretraining. Beyond vEM, it potentially provides a generalizable slice-to-3D solution across diverse imaging domains.