# Diffusion-Based Synthetic Brightfield Microscopy Images for Enhanced Single Cell Detection

arXiv: https://arxiv.org/abs/2512.00078 · HTML: https://mlsanity.com/paper/2512.00078/

## Key facts

- No citing papers in the map yet (data through 14 Oct 2026).
- Builds on 10 of its 45 references that are in the map.
- First posted on arXiv 25 Nov 2025.

## Builds on (most cited first, 10 of 10)

- [Denoising Diffusion Probabilistic Models](https://mlsanity.com/paper/2006.11239/) (2020)
- [U-Net: Convolutional Networks for Biomedical Image Segmentation](https://mlsanity.com/paper/1505.04597/) (2015)
- [Microsoft COCO: Common Objects in Context](https://mlsanity.com/paper/1405.0312/) (2014)
- [Denoising Diffusion Implicit Models](https://mlsanity.com/paper/2010.02502/) (2020)
- [Deep Unsupervised Learning using Nonequilibrium Thermodynamics](https://mlsanity.com/paper/1503.03585/) (2015)
- [DETRs Beat YOLOs on Real-time Object Detection](https://mlsanity.com/paper/2304.08069/) (2023)
- [YOLOv9: Learning What You Want to Learn Using Programmable Gradient Information](https://mlsanity.com/paper/2402.13616/) (2024)
- [Combating Mode Collapse in GAN training: An Empirical Analysis using Hessian Eigenvalues](https://mlsanity.com/paper/2012.09673/) (2020)
- [Class-Guided Image-to-Image Diffusion: Cell Painting from Brightfield Images with Class Labels](https://mlsanity.com/paper/2303.08863/) (2023)
- [Conditional Variational Diffusion Models](https://mlsanity.com/paper/2312.02246/) (2023)

## Abstract (arXiv)

Accurate single cell detection in brightfield microscopy is crucial for biological research, yet data scarcity and annotation bottlenecks limit the progress of deep learning methods. We investigate the use of unconditional models to generate synthetic brightfield microscopy images and evaluate their impact on object detection performance. A U-Net based diffusion model was trained and used to create datasets with varying ratios of synthetic and real images. Experiments with YOLOv8, YOLOv9 and RT-DETR reveal that training with synthetic data can achieve improved detection accuracies (at minimal costs). A human expert survey demonstrates the high realism of generated images, with experts not capable to distinguish them from real microscopy images (accuracy 50%). Our findings suggest that diffusion-based synthetic data generation is a promising avenue for augmenting real datasets in microscopy image analysis, reducing the reliance on extensive manual annotation and potentially improving the robustness of cell detection models.