# Prompt-DAS: Annotation-Efficient Prompt Learning for Domain Adaptive Semantic Segmentation of Electron Microscopy Images

arXiv: https://arxiv.org/abs/2509.18973 · HTML: https://mlsanity.com/paper/2509.18973/

## Key facts

- Cited by 1 papers in the map (data through 14 Oct 2026).
- Builds on 12 of its 20 references that are in the map.
- First posted on arXiv 23 Sep 2025.

## Builds on (most cited first, 12 of 12)

- [An Image is Worth 16x16 Words: Transformers for Image Recognition at Scale](https://mlsanity.com/paper/2010.11929/) (2020)
- [U-Net: Convolutional Networks for Biomedical Image Segmentation](https://mlsanity.com/paper/1505.04597/) (2015)
- [Segment Anything](https://mlsanity.com/paper/2304.02643/) (2023)
- [Emerging Properties in Self-Supervised Vision Transformers](https://mlsanity.com/paper/2104.14294/) (2021)
- [Panoptic Segmentation](https://mlsanity.com/paper/1801.00868/) (2018)
- [Medical SAM Adapter: Adapting Segment Anything Model for Medical Image Segmentation](https://mlsanity.com/paper/2304.12620/) (2023)
- [DN-DETR: Accelerate DETR Training by Introducing Query DeNoising](https://mlsanity.com/paper/2203.01305/) (2022)
- [SAM-Med2D](https://mlsanity.com/paper/2308.16184/) (2023)
- [Improving the Generalization of Segmentation Foundation Model under Distribution Shift via Weakly Supervised Adaptation](https://mlsanity.com/paper/2312.03502/) (2023)
- [Self-Prompt SAM: Medical Image Segmentation via Automatic Prompt SAM Adaptation](https://mlsanity.com/paper/2502.00630/) (2025)
- [WDA-Net: Weakly-Supervised Domain Adaptive Segmentation of Electron Microscopy](https://mlsanity.com/paper/2210.13109/) (2022)
- [Weakly-Supervised Cross-Domain Segmentation of Electron Microscopy with Sparse Point Annotation](https://mlsanity.com/paper/2404.00667/) (2024)

## Built on by (most cited first, 1 of 1)

- [Prefer-DAS: Learning from Local Preferences and Sparse Prompts for Domain Adaptive Segmentation of Electron Microscopy](https://mlsanity.com/paper/2602.19423/) (2026)

## Abstract (arXiv)

Domain adaptive segmentation (DAS) of numerous organelle instances from large-scale electron microscopy (EM) is a promising way to enable annotation-efficient learning. Inspired by SAM, we propose a promptable multitask framework, namely Prompt-DAS, which is flexible enough to utilize any number of point prompts during the adaptation training stage and testing stage. Thus, with varying prompt configurations, Prompt-DAS can perform unsupervised domain adaptation (UDA) and weakly supervised domain adaptation (WDA), as well as interactive segmentation during testing. Unlike the foundation model SAM, which necessitates a prompt for each individual object instance, Prompt-DAS is only trained on a small dataset and can utilize full points on all instances, sparse points on partial instances, or even no points at all, facilitated by the incorporation of an auxiliary center-point detection task. Moreover, a novel prompt-guided contrastive learning is proposed to enhance discriminative feature learning. Comprehensive experiments conducted on challenging benchmarks demonstrate the effectiveness of the proposed approach over existing UDA, WDA, and SAM-based approaches.