# Reconstructing Heterogeneous Biomolecules via Hierarchical Gaussian Mixtures and Part Discovery

arXiv: https://arxiv.org/abs/2506.09063 · HTML: https://mlsanity.com/paper/2506.09063/

## Key facts

- Cited by 1 papers in the map (data through 14 Oct 2026).
- Builds on 15 of its 47 references that are in the map.
- First posted on arXiv 6 Jun 2025.

## Builds on (most cited first, 15 of 15)

- [3D Gaussian Splatting for Real-Time Radiance Field Rendering](https://mlsanity.com/paper/2308.04079/) (2023)
- [DeepSDF: Learning Continuous Signed Distance Functions for Shape Representation](https://mlsanity.com/paper/1901.05103/) (2019)
- [D-NeRF: Neural Radiance Fields for Dynamic Scenes](https://mlsanity.com/paper/2011.13961/) (2020)
- [Nerfies: Deformable Neural Radiance Fields](https://mlsanity.com/paper/2011.12948/) (2020)
- [4D Gaussian Splatting for Real-Time Dynamic Scene Rendering](https://mlsanity.com/paper/2310.08528/) (2023)
- [Deformable 3D Gaussians for High-Fidelity Monocular Dynamic Scene Reconstruction](https://mlsanity.com/paper/2309.13101/) (2023)
- [Dynamic 3D Gaussians: Tracking by Persistent Dynamic View Synthesis](https://mlsanity.com/paper/2308.09713/) (2023)
- [Scaffold-GS: Structured 3D Gaussians for View-Adaptive Rendering](https://mlsanity.com/paper/2312.00109/) (2023)
- [Mip-Splatting: Alias-free 3D Gaussian Splatting](https://mlsanity.com/paper/2311.16493/) (2023)
- [SuGaR: Surface-Aligned Gaussian Splatting for Efficient 3D Mesh Reconstruction and High-Quality Mesh Rendering](https://mlsanity.com/paper/2311.12775/) (2023)
- [3D Gaussian Splatting as Markov Chain Monte Carlo](https://mlsanity.com/paper/2404.09591/) (2024)
- [Per-Gaussian Embedding-Based Deformation for Deformable 3D Gaussian Splatting](https://mlsanity.com/paper/2404.03613/) (2024)
- [Amortized Inference for Heterogeneous Reconstruction in Cryo-EM](https://mlsanity.com/paper/2210.07387/) (2022)
- [CryoBench: Diverse and challenging datasets for the heterogeneity problem in cryo-EM](https://mlsanity.com/paper/2408.05526/) (2024)
- [Building Proteins in a Day: Efficient 3D Molecular Reconstruction](https://mlsanity.com/paper/1504.03573/) (2015)

## Built on by (most cited first, 1 of 1)

- [CryoSplat: Gaussian Splatting for Cryo-EM Homogeneous Reconstruction](https://mlsanity.com/paper/2508.04929/) (2025)

## Abstract (arXiv)

Cryo-EM is a transformational paradigm in molecular biology where computational methods are used to infer 3D molecular structure at atomic resolution from extremely noisy 2D electron microscope images. At the forefront of research is how to model the structure when the imaged particles exhibit non-rigid conformational flexibility and compositional variation where parts are sometimes missing. We introduce a novel 3D reconstruction framework with a hierarchical Gaussian mixture model, inspired in part by Gaussian Splatting for 4D scene reconstruction. In particular, the structure of the model is grounded in an initial process that infers a part-based segmentation of the particle, providing essential inductive bias in order to handle both conformational and compositional variability. The framework, called CryoSPIRE, is shown to reveal biologically meaningful structures on complex experimental datasets, and establishes a new state-of-the-art on CryoBench, a benchmark for cryo-EM heterogeneity methods.