# Tiling artifacts and trade-offs of feature normalization in the segmentation of large biological images

arXiv: https://arxiv.org/abs/2503.19545 · HTML: https://mlsanity.com/paper/2503.19545/

## Key facts

- Cited by 4 papers in the map (data through 14 Oct 2026).
- Builds on 10 of its 25 references that are in the map.
- First posted on arXiv 25 Mar 2025.

## Builds on (most cited first, 10 of 10)

- [Segment Anything](https://mlsanity.com/paper/2304.02643/) (2023)
- [Batch Normalization: Accelerating Deep Network Training by Reducing Internal Covariate Shift](https://mlsanity.com/paper/1502.03167/) (2015)
- [The Cityscapes Dataset for Semantic Urban Scene Understanding](https://mlsanity.com/paper/1604.01685/) (2016)
- [3D U-Net: Learning Dense Volumetric Segmentation from Sparse Annotation](https://mlsanity.com/paper/1606.06650/) (2016)
- [Understanding the Effective Receptive Field in Deep Convolutional Neural Networks](https://mlsanity.com/paper/1701.04128/) (2017)
- [Batch Renormalization: Towards Reducing Minibatch Dependence in Batch-Normalized Models](https://mlsanity.com/paper/1702.03275/) (2017)
- [Normalization Techniques in Training DNNs: Methodology, Analysis and Application](https://mlsanity.com/paper/2009.12836/) (2020)
- [Star-convex Polyhedra for 3D Object Detection and Segmentation in Microscopy](https://mlsanity.com/paper/1908.03636/) (2019)
- [Tiling and Stitching Segmentation Output for Remote Sensing: Basic Challenges and Recommendations](https://mlsanity.com/paper/1805.12219/) (2018)
- [How Shift Equivariance Impacts Metric Learning for Instance Segmentation](https://mlsanity.com/paper/2101.05846/) (2021)

## Built on by (most cited first, 4 of 4)

- [NeuroSeg Meets DINOv3: Transferring 2D Self-Supervised Visual Priors to 3D Neuron Segmentation via DINOv3 Initialization](https://mlsanity.com/paper/2603.23104/) (2026)
- [IConE: Batch Independent Collapse Prevention for Self-Supervised Representation Learning](https://mlsanity.com/paper/2603.15263/) (2026)
- [Stitching and dimensionality effects on large artificially generated volume datasets](https://mlsanity.com/paper/2606.20095/) (2026)
- [SWITi: Quantifying and Reducing Tiling Artifacts with Sliding Window Inner Tiling](https://mlsanity.com/paper/2607.18990/) (2026)

## Abstract (arXiv)

Segmentation of very large images is a common problem in microscopy, medical imaging or remote sensing. The problem is usually addressed by sliding window inference, which can theoretically lead to seamlessly stitched predictions. However, in practice many of the popular pipelines still suffer from tiling artifacts. We investigate the root cause of these issues and show that they stem from the normalization layers within the neural networks. We propose indicators to detect normalization issues and further explore the trade-offs between artifact-free and high-quality predictions, using three diverse microscopy datasets as examples. Finally, we propose to use BatchRenorm as the most suitable normalization strategy, which effectively removes tiling artifacts and enhances transfer performance, thereby improving the reusability of trained networks for new datasets.