# Diffusion-driven lensless fiber endomicroscopic quantitative phase imaging towards digital pathology

arXiv: https://arxiv.org/abs/2407.18456 · HTML: https://mlsanity.com/paper/2407.18456/

## Key facts

- No citing papers in the map yet (data through 14 Oct 2026).
- Builds on 18 of its 68 references that are in the map.
- First posted on arXiv 26 Jul 2024.

## Builds on (most cited first, 18 of 18)

- [Adam: A Method for Stochastic Optimization](https://mlsanity.com/paper/1412.6980/) (2014)
- [Denoising Diffusion Probabilistic Models](https://mlsanity.com/paper/2006.11239/) (2020)
- [U-Net: Convolutional Networks for Biomedical Image Segmentation](https://mlsanity.com/paper/1505.04597/) (2015)
- [High-Resolution Image Synthesis with Latent Diffusion Models](https://mlsanity.com/paper/2112.10752/) (2021)
- [Segment Anything](https://mlsanity.com/paper/2304.02643/) (2023)
- [Generative Adversarial Networks](https://mlsanity.com/paper/2203.00667/) (2022)
- [Adding Conditional Control to Text-to-Image Diffusion Models](https://mlsanity.com/paper/2302.05543/) (2023)
- [Fashion-MNIST: a Novel Image Dataset for Benchmarking Machine Learning Algorithms](https://mlsanity.com/paper/1708.07747/) (2017)
- [Generative Modeling by Estimating Gradients of the Data Distribution](https://mlsanity.com/paper/1907.05600/) (2019)
- [A Survey on Visual Transformer](https://mlsanity.com/paper/2012.12556/) (2020)
- [Watch your Up-Convolution: CNN Based Generative Deep Neural Networks are Failing to Reproduce Spectral Distributions](https://mlsanity.com/paper/2003.01826/) (2020)
- [Equivariant 3D-Conditional Diffusion Models for Molecular Linker Design](https://mlsanity.com/paper/2210.05274/) (2022)
- [Deep Phase Decoder: Self-calibrating phase microscopy with an untrained deep neural network](https://mlsanity.com/paper/2001.09803/) (2020)
- [A Diffusion Model Predicts 3D Shapes from 2D Microscopy Images](https://mlsanity.com/paper/2208.14125/) (2022)
- [Latent Space Diffusion Models of Cryo-EM Structures](https://mlsanity.com/paper/2211.14169/) (2022)
- [Quantitative phase imaging through an ultra-thin lensless fiber endoscope](https://mlsanity.com/paper/2112.12055/) (2021)
- [Calibration-free quantitative phase imaging in multi-core fiber endoscopes using end-to-end deep learning](https://mlsanity.com/paper/2312.07102/) (2023)
- [AI-driven projection tomography with multicore fibre-optic cell rotation](https://mlsanity.com/paper/2312.07631/) (2023)

## Abstract (arXiv)

Lensless fiber endomicroscope is an emerging tool for in-vivo microscopic imaging, where quantitative phase imaging (QPI) can be utilized as a label-free method to enhance image contrast. However, existing single-shot phase reconstruction methods through lensless fiber endomicroscope typically perform well on simple images but struggle with complex microscopic structures. Here, we propose a speckle-conditioned diffusion model (SpecDiffusion), which reconstructs phase images directly from speckles captured at the detection side of a multi-core fiber (MCF). Unlike conventional neural networks, SpecDiffusion employs iterative phase denoising steps for speckle-driven phase reconstruction. The iteration scheme allows SpecDiffusion to break down the phase reconstruction process into multiple steps, gradually building up to the final phase image. This attribute alleviates the computation challenge at each step and enables the reconstruction of rich details in complex microscopic images. To validate its efficacy, we build an optical system to capture speckles from MCF and construct a dataset consisting of 100,000 paired images. SpecDiffusion provides high-fidelity phase reconstruction results and shows powerful generalization capacity for unseen objects, such as test charts and biological tissues, reducing the average mean absolute error of the reconstructed tissue images by 7 times. Furthermore, the reconstructed tissue images using SpecDiffusion shows higher accuracy in zero-shot cell segmentation tasks compared to the conventional method, demonstrating the potential for further cell morphology analysis through the learning-based lensless fiber endomicroscope. SpecDiffusion offers a precise and generalized method to phase reconstruction through scattering media, including MCFs, opening new perspective in lensless fiber endomicroscopic imaging.