# Feature-prompting GBMSeg: One-Shot Reference Guided Training-Free Prompt Engineering for Glomerular Basement Membrane Segmentation

arXiv: https://arxiv.org/abs/2406.16271 · HTML: https://mlsanity.com/paper/2406.16271/

## Key facts

- Cited by 5 papers in the map (data through 14 Oct 2026).
- Builds on 9 of its 21 references that are in the map.
- First posted on arXiv 24 Jun 2024.

## Builds on (most cited first, 9 of 9)

- [Learning Transferable Visual Models From Natural Language Supervision](https://mlsanity.com/paper/2103.00020/) (2021)
- [Segment Anything](https://mlsanity.com/paper/2304.02643/) (2023)
- [DINOv2: Learning Robust Visual Features without Supervision](https://mlsanity.com/paper/2304.07193/) (2023)
- [Scaling Up Visual and Vision-Language Representation Learning With Noisy Text Supervision](https://mlsanity.com/paper/2102.05918/) (2021)
- [Personalize Segment Anything Model with One Shot](https://mlsanity.com/paper/2305.03048/) (2023)
- [Hypercorrelation Squeeze for Few-Shot Segmentation](https://mlsanity.com/paper/2104.01538/) (2021)
- [Matcher: Segment Anything with One Shot Using All-Purpose Feature Matching](https://mlsanity.com/paper/2305.13310/) (2023)
- [Cost Aggregation with 4D Convolutional Swin Transformer for Few-Shot Segmentation](https://mlsanity.com/paper/2207.10866/) (2022)
- [CleftNet: Augmented Deep Learning for Synaptic Cleft Detection from Brain Electron Microscopy](https://mlsanity.com/paper/2101.04266/) (2021)

## Built on by (most cited first, 5 of 5)

- [Prompt Engineering in Segment Anything Model: Methodologies, Applications, and Emerging Challenges](https://mlsanity.com/paper/2507.09562/) (2025)
- [Prompt Mechanisms in Medical Imaging: A Comprehensive Survey](https://mlsanity.com/paper/2507.01055/) (2025)
- [Attack for Defense: Adversarial Agents for Point Prompt Optimization Empowering Segment Anything Model](https://mlsanity.com/paper/2509.18891/) (2025)
- [How Close Are We? Limitations and Progress of AI Models in Banff Lesion Scoring](https://mlsanity.com/paper/2510.27158/) (2025)
- [Beyond Isotropic Assumptions: Continuity-Constrained Segmentation and GPU Morphometry for Nanoscale GBM Analysis](https://mlsanity.com/paper/2608.07575/) (2026)

## Abstract (arXiv)

Assessment of the glomerular basement membrane (GBM) in transmission electron microscopy (TEM) is crucial for diagnosing chronic kidney disease (CKD). The lack of domain-independent automatic segmentation tools for the GBM necessitates an AI-based solution to automate the process. In this study, we introduce GBMSeg, a training-free framework designed to automatically segment the GBM in TEM images guided only by a one-shot annotated reference. Specifically, GBMSeg first exploits the robust feature matching capabilities of the pretrained foundation model to generate initial prompt points, then introduces a series of novel automatic prompt engineering techniques across the feature and physical space to optimize the prompt scheme. Finally, GBMSeg employs a class-agnostic foundation segmentation model with the generated prompt scheme to obtain accurate segmentation results. Experimental results on our collected 2538 TEM images confirm that GBMSeg achieves superior segmentation performance with a Dice similarity coefficient (DSC) of 87.27% using only one labeled reference image in a training-free manner, outperforming recently proposed one-shot or few-shot methods. In summary, GBMSeg introduces a distinctive automatic prompt framework that facilitates robust domain-independent segmentation performance without training, particularly advancing the automatic prompting of foundation segmentation models for medical images. Future work involves automating the thickness measurement of segmented GBM and quantifying pathological indicators, holding significant potential for advancing pathology assessments in clinical applications. The source code is available on https://github.com/SnowRain510/GBMSeg