# Diffusion-based generation of Histopathological Whole Slide Images at a Gigapixel scale

arXiv: https://arxiv.org/abs/2311.08199 · HTML: https://mlsanity.com/paper/2311.08199/

## Key facts

- Cited by 10 papers in the map (data through 14 Oct 2026).
- Placed in Medical imaging and segmentation, one of 14 directions in Diffusion models.
- Builds on 32 of its 47 references that are in the map.
- First posted on arXiv 14 Nov 2023.

## Placed in

- Diffusion models / [Medical imaging and segmentation](https://mlsanity.com/diffusion/medical-imaging-and-segmentation/)

## Builds on (most cited first, 30 of 32)

- [Attention Is All You Need](https://mlsanity.com/paper/1706.03762/) (2017)
- [Denoising Diffusion Probabilistic Models](https://mlsanity.com/paper/2006.11239/) (2020)
- [High-Resolution Image Synthesis with Latent Diffusion Models](https://mlsanity.com/paper/2112.10752/) (2021)
- [Denoising Diffusion Implicit Models](https://mlsanity.com/paper/2010.02502/) (2020)
- [Score-Based Generative Modeling through Stochastic Differential Equations](https://mlsanity.com/paper/2011.13456/) (2020)
- [Diffusion Models Beat GANs on Image Synthesis](https://mlsanity.com/paper/2105.05233/) (2021)
- [Deep Unsupervised Learning using Nonequilibrium Thermodynamics](https://mlsanity.com/paper/1503.03585/) (2015)
- [Photorealistic Text-to-Image Diffusion Models with Deep Language Understanding](https://mlsanity.com/paper/2205.11487/) (2022)
- [Rethinking the Inception Architecture for Computer Vision](https://mlsanity.com/paper/1512.00567/) (2015)
- [Improved Denoising Diffusion Probabilistic Models](https://mlsanity.com/paper/2102.09672/) (2021)
- [Elucidating the Design Space of Diffusion-Based Generative Models](https://mlsanity.com/paper/2206.00364/) (2022)
- [Unsupervised Representation Learning with Deep Convolutional Generative Adversarial Networks](https://mlsanity.com/paper/1511.06434/) (2015)
- [Consistency Models](https://mlsanity.com/paper/2303.01469/) (2023)
- [Enhanced Deep Residual Networks for Single Image Super-Resolution](https://mlsanity.com/paper/1707.02921/) (2017)
- [Image Super-Resolution via Iterative Refinement](https://mlsanity.com/paper/2104.07636/) (2021)
- [Cascaded Diffusion Models for High Fidelity Image Generation](https://mlsanity.com/paper/2106.15282/) (2021)
- [Diffusion Models in Vision: A Survey](https://mlsanity.com/paper/2209.04747/) (2022)
- [Improved Precision and Recall Metric for Assessing Generative Models](https://mlsanity.com/paper/1904.06991/) (2019)
- [Zero-Shot Image Restoration Using Denoising Diffusion Null-Space Model](https://mlsanity.com/paper/2212.00490/) (2022)
- [Blended Latent Diffusion](https://mlsanity.com/paper/2206.02779/) (2022)
- [Are GANs Created Equal? A Large-Scale Study](https://mlsanity.com/paper/1711.10337/) (2017)
- [Gotta Go Fast When Generating Data with Score-Based Models](https://mlsanity.com/paper/2105.14080/) (2021)
- [The Role of ImageNet Classes in Fr'echet Inception Distance](https://mlsanity.com/paper/2203.06026/) (2022)
- [Synthetic Data for Deep Learning](https://mlsanity.com/paper/1909.11512/) (2019)
- [PacGAN: The power of two samples in generative adversarial networks](https://mlsanity.com/paper/1712.04086/) (2017)
- [Normalization Techniques in Training DNNs: Methodology, Analysis and Application](https://mlsanity.com/paper/2009.12836/) (2020)
- [A Morphology Focused Diffusion Probabilistic Model for Synthesis of Histopathology Images](https://mlsanity.com/paper/2209.13167/) (2022)
- [Methods for Segmentation and Classification of Digital Microscopy Tissue Images](https://mlsanity.com/paper/1810.13230/) (2018)
- [Diffusion Models as Masked Autoencoders](https://mlsanity.com/paper/2304.03283/) (2023)
- [Selective Synthetic Augmentation with HistoGAN for Improved Histopathology Image Classification](https://mlsanity.com/paper/2111.06399/) (2021)

## Built on by (most cited first, 10 of 10)

- [ZoomLDM: Latent Diffusion Model for multi-scale image generation](https://mlsanity.com/paper/2411.16969/) (2024)
- [TopoCellGen: Generating Histopathology Cell Topology with a Diffusion Model](https://mlsanity.com/paper/2412.06011/) (2024)
- [Content Generation Models in Computational Pathology: A Comprehensive Survey on Methods, Applications, and Challenges](https://mlsanity.com/paper/2505.10993/) (2025)
- [PosDiffAE: Position-aware Diffusion Auto-encoder For High-Resolution Brain Tissue Classification Incorporating Artifact Restoration](https://mlsanity.com/paper/2507.02405/) (2025)
- [Mask-guided cross-image attention for zero-shot in-silico histopathologic image generation with a diffusion model](https://mlsanity.com/paper/2407.11664/) (2024)
- [Advancing Histopathology with Deep Learning Under Data Scarcity: A Decade in Review](https://mlsanity.com/paper/2410.19820/) (2024)
- [Computationally Efficient Diffusion Models in Medical Imaging: A Comprehensive Review](https://mlsanity.com/paper/2505.07866/) (2025)
- [MSDM: Generating Task-Specific Pathology Images with a Multimodal Conditioned Diffusion Model for Cell and Nuclei Segmentation](https://mlsanity.com/paper/2510.09121/) (2025)
- [Layout-Guided Controllable Pathology Image Generation with In-Context Diffusion Transformers](https://mlsanity.com/paper/2603.13386/) (2026)
- [BigCell: Generating Gigapixel Whole-Slide Images](https://mlsanity.com/paper/2610.14261/) (2026)

## Abstract (arXiv)

We present a novel diffusion-based approach to generate synthetic histopathological Whole Slide Images (WSIs) at an unprecedented gigapixel scale. Synthetic WSIs have many potential applications: They can augment training datasets to enhance the performance of many computational pathology applications. They allow the creation of synthesized copies of datasets that can be shared without violating privacy regulations. Or they can facilitate learning representations of WSIs without requiring data annotations. Despite this variety of applications, no existing deep-learning-based method generates WSIs at their typically high resolutions. Mainly due to the high computational complexity. Therefore, we propose a novel coarse-to-fine sampling scheme to tackle image generation of high-resolution WSIs. In this scheme, we increase the resolution of an initial low-resolution image to a high-resolution WSI. Particularly, a diffusion model sequentially adds fine details to images and increases their resolution. In our experiments, we train our method with WSIs from the TCGA-BRCA dataset. Additionally to quantitative evaluations, we also performed a user study with pathologists. The study results suggest that our generated WSIs resemble the structure of real WSIs.