# Self-Supervised Super-Resolution Approach for Isotropic Reconstruction of 3D Electron Microscopy Images from Anisotropic Acquisition

arXiv: https://arxiv.org/abs/2309.10646 · HTML: https://mlsanity.com/paper/2309.10646/

## Key facts

- Cited by 1 papers in the map (data through 14 Oct 2026).
- Builds on 11 of its 21 references that are in the map.
- First posted on arXiv 19 Sep 2023.

## Builds on (most cited first, 11 of 11)

- [Adam: A Method for Stochastic Optimization](https://mlsanity.com/paper/1412.6980/) (2014)
- [Swin Transformer: Hierarchical Vision Transformer using Shifted Windows](https://mlsanity.com/paper/2103.14030/) (2021)
- [SwinIR: Image Restoration Using Swin Transformer](https://mlsanity.com/paper/2108.10257/) (2021)
- [Uformer: A General U-Shaped Transformer for Image Restoration](https://mlsanity.com/paper/2106.03106/) (2021)
- ["Zero-Shot" Super-Resolution using Deep Internal Learning](https://mlsanity.com/paper/1712.06087/) (2017)
- [Learning a Single Convolutional Super-Resolution Network for Multiple Degradations](https://mlsanity.com/paper/1712.06116/) (2017)
- [Real-World Single Image Super-Resolution: A Brief Review](https://mlsanity.com/paper/2103.02368/) (2021)
- [Projected Distribution Loss for Image Enhancement](https://mlsanity.com/paper/2012.09289/) (2020)
- [Isotropic reconstruction of 3D fluorescence microscopy images using convolutional neural networks](https://mlsanity.com/paper/1704.01510/) (2017)
- [Deep Learning for Isotropic Super-Resolution from Non-Isotropic 3D Electron Microscopy](https://mlsanity.com/paper/1706.03142/) (2017)
- [Multimodal Image Super-resolution via Joint Sparse Representations induced by Coupled Dictionaries](https://mlsanity.com/paper/1709.08680/) (2017)

## Built on by (most cited first, 1 of 1)

- [VEMamba: Efficient Isotropic Reconstruction of Volume Electron Microscopy with Axial-Lateral Consistent Mamba](https://mlsanity.com/paper/2603.00887/) (2026)

## Abstract (arXiv)

Three-dimensional electron microscopy (3DEM) is an essential technique to investigate volumetric tissue ultra-structure. Due to technical limitations and high imaging costs, samples are often imaged anisotropically, where resolution in the axial direction (z) is lower than in the lateral directions (x,y). This anisotropy 3DEM can hamper subsequent analysis and visualization tasks. To overcome this limitation, we propose a novel deep-learning (DL)-based self-supervised super-resolution approach that computationally reconstructs isotropic 3DEM from the anisotropic acquisition. The proposed DL-based framework is built upon the U-shape architecture incorporating vision-transformer (ViT) blocks, enabling high-capability learning of local and global multi-scale image dependencies. To train the tailored network, we employ a self-supervised approach. Specifically, we generate pairs of anisotropic and isotropic training datasets from the given anisotropic 3DEM data. By feeding the given anisotropic 3DEM dataset in the trained network through our proposed framework, the isotropic 3DEM is obtained. Importantly, this isotropic reconstruction approach relies solely on the given anisotropic 3DEM dataset and does not require pairs of co-registered anisotropic and isotropic 3DEM training datasets. To evaluate the effectiveness of the proposed method, we conducted experiments using three 3DEM datasets acquired from brain. The experimental results demonstrated that our proposed framework could successfully reconstruct isotropic 3DEM from the anisotropic acquisition.