# Ins-ATP: Deep Estimation of ATP for Organoid Based on High Throughput Microscopic Images

arXiv: https://arxiv.org/abs/2303.06796 · HTML: https://mlsanity.com/paper/2303.06796/

## Key facts

- Cited by 1 papers in the map (data through 14 Oct 2026).
- Builds on 21 of its 36 references that are in the map.
- First posted on arXiv 13 Mar 2023.

## Builds on (most cited first, 21 of 21)

- [Attention Is All You Need](https://mlsanity.com/paper/1706.03762/) (2017)
- [Deep Residual Learning for Image Recognition](https://mlsanity.com/paper/1512.03385/) (2015)
- [PyTorch: An Imperative Style, High-Performance Deep Learning Library](https://mlsanity.com/paper/1912.01703/) (2019)
- [U-Net: Convolutional Networks for Biomedical Image Segmentation](https://mlsanity.com/paper/1505.04597/) (2015)
- [Very Deep Convolutional Networks for Large-Scale Image Recognition](https://mlsanity.com/paper/1409.1556/) (2014)
- [Faster R-CNN: Towards Real-Time Object Detection with Region Proposal Networks](https://mlsanity.com/paper/1506.01497/) (2015)
- [End-to-End Object Detection with Transformers](https://mlsanity.com/paper/2005.12872/) (2020)
- [You Only Look Once: Unified, Real-Time Object Detection](https://mlsanity.com/paper/1506.02640/) (2015)
- [Going Deeper with Convolutions](https://mlsanity.com/paper/1409.4842/) (2014)
- [DeepLab: Semantic Image Segmentation with Deep Convolutional Nets, Atrous Convolution, and Fully Connected CRFs](https://mlsanity.com/paper/1606.00915/) (2016)
- [Inception-v4, Inception-ResNet and the Impact of Residual Connections on Learning](https://mlsanity.com/paper/1602.07261/) (2016)
- [Image Super-Resolution Using Deep Convolutional Networks](https://mlsanity.com/paper/1501.00092/) (2014)
- [A Survey on Deep Learning in Medical Image Analysis](https://mlsanity.com/paper/1702.05747/) (2017)
- [Dual Attention Network for Scene Segmentation](https://mlsanity.com/paper/1809.02983/) (2018)
- [Accelerating the Super-Resolution Convolutional Neural Network](https://mlsanity.com/paper/1608.00367/) (2016)
- [MaX-DeepLab: End-to-End Panoptic Segmentation with Mask Transformers](https://mlsanity.com/paper/2012.00759/) (2020)
- [Multiple Instance Learning: A Survey of Problem Characteristics and Applications](https://mlsanity.com/paper/1612.03365/) (2016)
- [Transformer for Single Image Super-Resolution](https://mlsanity.com/paper/2108.11084/) (2021)
- [Deep Learning in Medical Image Registration: A Survey](https://mlsanity.com/paper/1903.02026/) (2019)
- [Attentional Feature Fusion](https://mlsanity.com/paper/2009.14082/) (2020)
- [Weakly supervised multiple instance learning histopathological tumor segmentation](https://mlsanity.com/paper/2004.05024/) (2020)

## Built on by (most cited first, 1 of 1)

- [Spatio-Temporal Analysis of Patient-Derived Organoid Videos Using Deep Learning for the Prediction of Drug Efficacy](https://mlsanity.com/paper/2308.14461/) (2023)

## Abstract (arXiv)

Adenosine triphosphate (ATP) is a high-energy phosphate compound and the most direct energy source in organisms. ATP is an essential biomarker for evaluating cell viability in biology. Researchers often use ATP bioluminescence to measure the ATP of organoid after drug to evaluate the drug efficacy. However, ATP bioluminescence has some limitations, leading to unreliable drug screening results. Performing ATP bioluminescence causes cell lysis of organoids, so it is impossible to observe organoids' long-term viability changes after medication continually. To overcome the disadvantages of ATP bioluminescence, we propose Ins-ATP, a non-invasive strategy, the first organoid ATP estimation model based on the high-throughput microscopic image. Ins-ATP directly estimates the ATP of organoids from high-throughput microscopic images, so that it does not influence the drug reactions of organoids. Therefore, the ATP change of organoids can be observed for a long time to obtain more stable results. Experimental results show that the ATP estimation by Ins-ATP is in good agreement with those determined by ATP bioluminescence. Specifically, the predictions of Ins-ATP are consistent with the results measured by ATP bioluminescence in the efficacy evaluation experiments of different drugs.