# Cross-domain Microscopy Cell Counting by Disentangled Transfer Learning

arXiv: https://arxiv.org/abs/2211.14638 · HTML: https://mlsanity.com/paper/2211.14638/

## Key facts

- Cited by 2 papers in the map (data through 14 Oct 2026).
- Builds on 10 of its 27 references that are in the map.
- First posted on arXiv 26 Nov 2022.

## Builds on (most cited first, 10 of 10)

- [U-Net: Convolutional Networks for Biomedical Image Segmentation](https://mlsanity.com/paper/1505.04597/) (2015)
- [Very Deep Convolutional Networks for Large-Scale Image Recognition](https://mlsanity.com/paper/1409.1556/) (2014)
- [Generative Adversarial Networks](https://mlsanity.com/paper/2203.00667/) (2022)
- [Fully Convolutional Networks for Semantic Segmentation](https://mlsanity.com/paper/1411.4038/) (2014)
- [Perceptual Losses for Real-Time Style Transfer and Super-Resolution](https://mlsanity.com/paper/1603.08155/) (2016)
- [Learning Transferable Features with Deep Adaptation Networks](https://mlsanity.com/paper/1502.02791/) (2015)
- [CSRNet: Dilated Convolutional Neural Networks for Understanding the Highly Congested Scenes](https://mlsanity.com/paper/1802.10062/) (2018)
- [Class-Agnostic Counting](https://mlsanity.com/paper/1811.00472/) (2018)
- [Count-ception: Counting by Fully Convolutional Redundant Counting](https://mlsanity.com/paper/1703.08710/) (2017)
- [People, Penguins and Petri Dishes: Adapting Object Counting Models To New Visual Domains And Object Types Without Forgetting](https://mlsanity.com/paper/1711.05586/) (2017)

## Built on by (most cited first, 2 of 2)

- [TFCounter:Polishing Gems for Training-Free Object Counting](https://mlsanity.com/paper/2405.02301/) (2024)
- [Towards Conditional Feature Alignment for Cross-Domain Counting](https://mlsanity.com/paper/2506.17137/) (2025)

## Abstract (arXiv)

Microscopy images from different imaging conditions, organs, and tissues often have numerous cells with various shapes on a range of backgrounds. As a result, designing a deep learning model to count cells in a source domain becomes precarious when transferring them to a new target domain. To address this issue, manual annotation costs are typically the norm when training deep learning-based cell counting models across different domains. In this paper, we propose a cross-domain cell counting approach that requires only weak human annotation efforts. Initially, we implement a cell counting network that disentangles domain-specific knowledge from domain-agnostic knowledge in cell images, where they pertain to the creation of domain style images and cell density maps, respectively. We then devise an image synthesis technique capable of generating massive synthetic images founded on a few target-domain images that have been labeled. Finally, we use a public dataset consisting of synthetic cells as the source domain, where no manual annotation cost is present, to train our cell counting network; subsequently, we transfer only the domain-agnostic knowledge to a new target domain of real cell images. By progressively refining the trained model using synthesized target-domain images and several real annotated ones, our proposed cross-domain cell counting method achieves good performance compared to state-of-the-art techniques that rely on fully annotated training images in the target domain. We evaluated the efficacy of our cross-domain approach on two target domain datasets of actual microscopy cells, demonstrating the feasibility of requiring annotations on only a few images in a new domain.