# HUNIS: High-Performance Unsupervised Nuclei Instance Segmentation

arXiv: https://arxiv.org/abs/2203.14887 · HTML: https://mlsanity.com/paper/2203.14887/

## Key facts

- Cited by 1 papers in the map (data through 14 Oct 2026).
- Builds on 8 of its 26 references that are in the map.
- First posted on arXiv 28 Mar 2022.

## Builds on (most cited first, 8 of 8)

- [HoVer-Net: Simultaneous Segmentation and Classification of Nuclei in Multi-Tissue Histology Images](https://mlsanity.com/paper/1812.06499/) (2018)
- [Attention Gated Networks: Learning to Leverage Salient Regions in Medical Images](https://mlsanity.com/paper/1808.08114/) (2018)
- [CIA-Net: Robust Nuclei Instance Segmentation with Contour-aware Information Aggregation](https://mlsanity.com/paper/1903.05358/) (2019)
- [Unsupervised Instance Segmentation in Microscopy Images via Panoptic Domain Adaptation and Task Re-weighting](https://mlsanity.com/paper/2005.02066/) (2020)
- [Self-Supervised Nuclei Segmentation in Histopathological Images Using Attention](https://mlsanity.com/paper/2007.08373/) (2020)
- [Instance-aware Self-supervised Learning for Nuclei Segmentation](https://mlsanity.com/paper/2007.11186/) (2020)
- [DARCNN: Domain Adaptive Region-based Convolutional Neural Network for Unsupervised Instance Segmentation in Biomedical Images](https://mlsanity.com/paper/2104.01325/) (2021)
- [Unsupervised Data-Driven Nuclei Segmentation For Histology Images](https://mlsanity.com/paper/2110.07147/) (2021)

## Built on by (most cited first, 1 of 1)

- [LG-NuSegHop: A Local-to-Global Self-Supervised Pipeline For Nuclei Instance Segmentation](https://mlsanity.com/paper/2511.04892/) (2025)

## Abstract (arXiv)

A high-performance unsupervised nuclei instance segmentation (HUNIS) method is proposed in this work. HUNIS consists of two-stage block-wise operations. The first stage includes: 1) adaptive thresholding of pixel intensities, 2) incorporation of nuclei size/shape priors and 3) removal of false positive nuclei instances. Then, HUNIS conducts the second stage segmentation by receiving guidance from the first one. The second stage exploits the segmentation masks obtained in the first stage and leverages color and shape distributions for a more accurate segmentation. The main purpose of the two-stage design is to provide pixel-wise pseudo-labels from the first to the second stage. This self-supervision mechanism is novel and effective. Experimental results on the MoNuSeg dataset show that HUNIS outperforms all other unsupervised methods by a substantial margin. It also has a competitive standing among state-of-the-art supervised methods.