# 3D Image Super-Resolution by fluorophore fluctuations and MA-TIRF Microscopy reconstruction (3D-COL0RME)

arXiv: https://arxiv.org/abs/2111.03170 · HTML: https://mlsanity.com/paper/2111.03170/

## Key facts

- Cited by 1 papers in the map (data through 14 Oct 2026).
- First posted on arXiv 4 Nov 2021.

## Built on by (most cited first, 1 of 1)

- [Fluctuation-based deconvolution in fluorescence microscopy using plug-and-play denoisers](https://mlsanity.com/paper/2303.11212/) (2023)

## Abstract (arXiv)

We propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications. Our approach, called 3D-COL0RME (3D - Covariance-based ℓ0 super-Resolution Microscopy with intensity Estimation) improves both lateral and axial resolution by combining sparsity-based modelling for precise molecule localisation and intensity estimation in the lateral plane with a 3D reconstruction procedure in the axial one using Multi-Angle TIRF (MA-TIRF). Differently from state-of-the-art approaches, 3D-COL0RME does not require the use of special equipment as it can be used with standard fluorophores. We validate 3D-COL0RME on simulated MA-TIRF blinking-type data and on challenging real MA-TIRF acquisitions, showing significant resolution improvements.