# Single-Shot 3D Widefield Fluorescence Imaging with a Computational Miniature Mesoscope

arXiv: https://arxiv.org/abs/2003.11994 · HTML: https://mlsanity.com/paper/2003.11994/

## Key facts

- Cited by 4 papers in the map (data through 14 Oct 2026).
- First posted on arXiv 26 Mar 2020.

## Built on by (most cited first, 4 of 4)

- [PS2F: Polarized Spiral Point Spread Function for Single-Shot 3D Sensing](https://mlsanity.com/paper/2207.00945/) (2022)
- [Diffractive lensless imaging with optimized Voronoi-Fresnel phase](https://mlsanity.com/paper/2109.13703/) (2021)
- [Physics-informed generative real-time lens-free imaging](https://mlsanity.com/paper/2403.07786/) (2024)
- [Integration of Communication and Computational Imaging](https://mlsanity.com/paper/2410.19415/) (2024)

## Abstract (arXiv)

Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes / miniscopes are inherently constrained by their limited space-bandwidth-product, shallow depth-of-field, and the inability to resolve 3D distributed emitters. Here, we present a Computational Miniature Mesoscope (CM2) that overcomes these bottlenecks and enables single-shot 3D imaging across an 8 × 7-mm2 field-of-view and 2.5-mm depth-of-field, achieving 7-μm lateral resolution and better than 200-μm axial resolution. Notably, the CM2 has a compact lightweight design that integrates a microlens array for imaging and an LED array for excitation in a single platform. Its expanded imaging capability is enabled by computational imaging that augments the optics by algorithms. We experimentally validate the mesoscopic 3D imaging capability on volumetrically distributed fluorescent beads and fibers. We further quantify the effects of bulk scattering and background fluorescence on phantom experiments.